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Protein concentration in mM


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#1 samita

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Posted 05 June 2017 - 07:38 AM

How to convert the protein concentation from ug/ul to mM?? if the protein mw is about 60kDa

 

best

 

Samita



#2 bob1

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Posted 05 June 2017 - 10:02 AM

Use the formulae you would for calculating concentration in moles - just rearrange them. I would do it like this:

 

mass=c*v: amount in ug = concentration(ug/ul) * number of ul you have

 

Then number of moles(n)=mass/molar mass

 

then convert back into a concentration using c=n/v

 

Note that you need to make sure your units are consistent!!!! molar mass is in g/mol.



#3 samita

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Posted 06 June 2017 - 11:06 AM

Protein size is 100 kd. Its not in g/mol

#4 bob1

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Posted 06 June 2017 - 12:52 PM

In that case you should look up the definition of a Dalton (hint:1 Da is 1 g/mol)






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