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Cycloheximide concentration


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#1 Doraid

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Posted 25 May 2017 - 10:59 AM

Hello,

I'm attempting pulse chase assay.

I concentration I'm looking to use is 25microgram/mL.

I have 14X 100mm dishes and each takes 10mL. Can I multiply 25μg/mL by 140 to get the concentration I need for each dish resulting in dissolving 3.5mg in 140mL?

Is that right, or did I make a mistake?



#2 bob1

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Posted 25 May 2017 - 01:58 PM

That is correct 3.5 mg in 140 ml will give you 25 ug/ml. However usually cycloheximide is prepared at 10 mg/ml and then diluted. It is a lot easier and more consistent to weigh a larger mass and then dilute, than it is to weigh small masses repeatedly.



#3 Doraid

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Posted 05 June 2017 - 02:47 PM

Thanks.






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