I have been using kits with columns to purify genomic DNA which I then use as a template for PCR for sequencing or T7E1/CelI mutation-detection assays. I accidentally ordered a kit which does not use columns but rather the older approach of chloroform and ethanol precipitation. I think it should be fine but then I wonder why I've been using spin columns... just for convenience?
Submit your paper to J Biol Methods today!
How clean do I need my DNA to be?
1 reply to this topic