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How clean do I need my DNA to be?


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#1 seanspotatobusiness

seanspotatobusiness

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Posted 18 May 2017 - 05:14 AM

I have been using kits with columns to purify genomic DNA which I then use as a template for PCR for sequencing or T7E1/CelI mutation-detection assays. I accidentally ordered a kit which does not use columns but rather the older approach of chloroform and ethanol precipitation. I think it should be fine but then I wonder why I've been using spin columns... just for convenience? 



#2 bob1

bob1

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Posted 18 May 2017 - 05:45 AM

Partly correct, convenience is one of the features; they also save time, which, if you are employed, is frequently the biggest cost for any experimental laboratory.






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