Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Using modified CFP and YFP plasmids for FRET assay

pAquaN1 Citrine-C1 gfp cfp yfp

  • Please log in to reply
1 reply to this topic

#1 maarts

maarts

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 11 May 2017 - 02:18 AM

Does anybody have plasmids pAquaN1 and Citrine-C1? I relied on Addgene but during checkout found out they don't have these pklasmids anymore. These plasmids contain modified yellow and cyan fluorescent protein genes which I need for FRET experiments. Will be happy for any suggestions



#2 labtastic

labtastic

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 196 posts
18
Good

Posted 11 May 2017 - 09:52 AM

I assume the genes are <1kb like traditional fluorescent proteins? If so, $150 each you can just order a (codon optimized) Gblock with restriction sites/homology ends for simple one-step cloning into your vector without the hassle of MTA's, streaking cells, growing up cells, miniprepping, making primers, PCRing, etc.

 

I am a big fan of what Addgene is doing...but if you're just after a particular gene (as opposed to a specific vector or cell line) it is often more convenient and cheaper (when factoring in labor and time) to order the Gblock to obtain the desired gene since downstream cloning is easier and you have the freedom to order whatever you wish without being restricted of what is publicaly available.


Edited by labtastic, 11 May 2017 - 02:48 PM.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.