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We need a way to see microRNA and DNA in vitro for reverse aging

laser spectroscopy

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#1 Fredreload

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Posted 16 April 2017 - 05:25 AM

Imagine being able to observe microRNA and DNA action in vitro, that, with the help of computer visualization, could help decode every mechanics in the body and eventually a way to reverse aging. From 20 second to 40 second of the video about attosecond laser presented.

 

So how exactly do we reverse aging?

 

First we need to understand the mechanics, we get a snapshot of the complete DNA genome(gene expression) of every cell in the body in the 30 years old and attempt to reverse every cell's DNA gene expression in the body to match it as we grow older, like when I become 50 years old. We do not need to modify the DNA, just the gene expression. This SALK article shows that it is possible to change the epigenetic marks(gene expression) by expressing the four factors in the cell for two days and have the cell's age slowly reverse back to a younger age, that is before it becomes a stem cell completely. The transgenic mice is modified to respond to an antibiotic that would turn on the four genes, but we cannot do that to our bodies, so we need the help from microRNA to change the gene expression. Now this article shows that it is possible to create induced pluripotent stem cell from microRNA, but as far as I've looked everywhere there really is not an article about microRNA changing gene expression, it's more of a myth how silencing mRNA with microRNA would results lead to a change in gene expression. Hormone changes gene expression, but for microRNA, it's a myth. Now this article suggests a transfection method to get microRNA into the cell using a virus, but I prefer the natural way in which microRNA normally enters a cell, this is yet another problem scientists do not understand, maybe with the help of attosecond laser we can understand the mechanics better.

 

So the goal is to inject an artificial microRNA that would revert all 50 years old cells' gene expression to match the 30 years old cells' gene expression, but how are we going to test it? My idea is through computer programming, microRNA are codes and it binds to mRNA to silence it and eventually leads to a change in gene expression, such calculations can be predicted with computer computations. I'll leave the rest to your thoughts an feedback. Thanks for taking the time to read this


Edited by Fredreload, 16 April 2017 - 05:26 AM.


#2 Fredreload

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Posted 03 May 2017 - 05:31 AM

Light sheet microscopy is on its way to visualize cell in vivo, correction, in vivo, here, at 250nm resolution


Edited by Fredreload, 03 May 2017 - 05:31 AM.


#3 Jon Moulton

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Posted 24 May 2017 - 10:51 AM

"I've looked everywhere there really is not an article about microRNA changing gene expression, it's more of a myth how silencing mRNA with microRNA would results lead to a change in gene expression"  There is an extensive literature on this topic.  Have you tried PubMed at NCBI? ncbi.nlm.nih.gov


Jon D. Moulton
Gene Tools, LLC
www.gene-tools.com

#4 Fredreload

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Posted 26 May 2017 - 04:52 AM

"I've looked everywhere there really is not an article about microRNA changing gene expression, it's more of a myth how silencing mRNA with microRNA would results lead to a change in gene expression"  There is an extensive literature on this topic.  Have you tried PubMed at NCBI? ncbi.nlm.nih.gov

Well, I know how hormone regulates gene expression by phosphorylating transcription factors and have these transcription factors enter the nucleus to change the gene expression. But how does microRNA changes gene expression if all it does is silencing mRNA? And how does silencing microRNA activates transcription factors? I might have missed something here, but do enlighten me if you know the answer :D



#5 Fredreload

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Posted 17 June 2017 - 06:17 AM

So now you can see microRNA at work. But it would not be of much help unless you know where it came from or in which tissue this microRNA is produced. If you can capture in molecular detail of the full body in 3D then we can trace and track each of the microRNA and cell like a movie. Of course this requires visualization technique to rule out the structures possibly with a database, identify and correlate based on similar findings of the molecules and essentially the entire body.

 

We figure it will be easier to test microRNA with a computer simulation then on live animals. We can test a variable microRNA without having to inject it into a live specimen each time.

 

P.S Work with dream groups, they are more experienced in scanning


Edited by Fredreload, 17 June 2017 - 06:31 AM.





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