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Where to gate with overlapping population?

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#1 seanspotatobusiness



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Posted 14 April 2017 - 06:28 AM

My fluorescent cells are only weakly fluorescent so they overlap with the non-fluorescent cells as in the below image. Would it be acceptable to move the gate to the left to get more of the fluorescent cells and then simply subtract 2% (or whatever value shows positive in the negative control) from all the values to obtain a good indication of the percentage of "stained" (they're not stained, they're expressing a fluorescent protein) cells?



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