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Monocyte dissociation


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#1 chloeo

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Posted 25 August 2004 - 12:39 AM

Hi. I am culturing monocytes obtained from fresh human blood and am having issues detaching them from the plasticware (pre coated with 4% albumin). I never seem to get all the cells off. Have tried EDTA, lignocaine and Gibco cell dissociation buffer. Can anyone tell me if it is normal to only recover a small amount of cells? Also is there a better way of dissociating them, i want to do FACS so can't use enzymes?

#2 Labuser

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Posted 06 June 2011 - 09:34 AM

Hi There, i am having a similar problem but with PBMC. When i defrost them for culturing they are all glued together, so i cannot dissociate to count them/culture. Does anybody have a clue? thanks, livia




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