1 reply to this topic

[chloeo]

Hi. I am culturing monocytes obtained from fresh human blood and am having issues detaching them from the plasticware (pre coated with 4% albumin). I never seem to get all the cells off. Have tried EDTA, lignocaine and Gibco cell dissociation buffer. Can anyone tell me if it is normal to only recover a small amount of cells? Also is there a better way of dissociating them, i want to do FACS so can't use enzymes?

[Labuser]

Hi There, i am having a similar problem but with PBMC. When i defrost them for culturing they are all glued together, so i cannot dissociate to count them/culture. Does anybody have a clue? thanks, livia