Hi. I am culturing monocytes obtained from fresh human blood and am having issues detaching them from the plasticware (pre coated with 4% albumin). I never seem to get all the cells off. Have tried EDTA, lignocaine and Gibco cell dissociation buffer. Can anyone tell me if it is normal to only recover a small amount of cells? Also is there a better way of dissociating them, i want to do FACS so can't use enzymes?
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