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Question about transfecting siRNA into cells


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#1 MrChemMDPhD

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Posted 17 March 2017 - 08:53 AM

Hello everyone,

 

I have a quick question.  When adding the siRNA complex to the cells, what kind of media do we keep the cells in during transfection.  Someone told me to add just opti-mem to the cells and transfect in those for 6 hours, and then replace the opti-mem with regular growth media since siRNA will already be in the cells.  The company tech support told me to add 20% opti-mem to DMEM and transfect.

 

 

Not really sure what to do..

 

 

Thanks!



#2 merlav

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Posted 17 March 2017 - 10:38 AM

At the lab we use optimum and transfect the cells for a period of time and then replace the optimem with the regular media


Science without religion is lame, religion without science is blind.
Albert Einstein

I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale.
Marie Curie




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