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Lysis buffer preparation

DNA extraction buffer solution Lysis Preparation

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#1 Lysis buffer preparation

Lysis buffer preparation


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Posted 27 February 2017 - 11:34 PM

Hi! I've to prepare 50ml of (Tris0.1M pH8/EDTA 0.1M/ NaCl 0.15M/1%SDS/2%Triton 100x) and I would like to know which is the correct order to add the reactives and prepare it.


Could you help me?

#2 mdfenko


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Posted 28 February 2017 - 05:04 AM

in general, you can add them in any order. however, you should probably add the edta after the tris to ensure that it remains in solution. also, don't mix too vigorously after adding the sds and triton or you'll produce foam. i prefer adding detergents to the solution last.

Edited by mdfenko, 28 February 2017 - 05:05 AM.

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