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Optimal dNTP storage pH


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#1 Vyse

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Posted 24 February 2017 - 01:57 PM

Ive been doing some reading up on dNTPs from various manufacturers. Nearly all suggest a storage pH range of between 7-8. It makes sense that you do not want a pH that is too low as it would increase the likelihood of hydrolysis reactions causing the formation of dNMP.

 

However, why would pH above 8 causes issues with dNTP storage?

 

Secondly, if I wanted to stabilise dNTPs for storage at temperatures in the 20-30c range what would be the most suitable method for achieving this without suffering loss of performance?

 

 

Thank You

 

V

 

 

 



#2 merlav

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Posted 03 March 2017 - 11:53 AM

If you need room temperature PCR reagents maybe a better approach are the PCR beads.  GE health and science have it.  I used several years ago and was easy (add water and DNA) and the performance is high quality.  


Science without religion is lame, religion without science is blind.
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I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale.
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