Primary epithelial cell line
Posted 24 August 2004 - 05:48 AM
Can anyone help me?
I have to establish a primary cell line consisting of epithelial cells. Does somebody know a protocol for removing fibroblast cells from mucous epithelial tissue? I won't have much starting material and I would like to be able to keep them as a line for as much as 15 passages.
Thanks a bunch
Posted 30 August 2004 - 10:00 PM
Furthermore, do regular "cell seeding", epithelial cells will be faster to attach to the bottom of dish than fibroblasts.
By repeating these procedures, you can get a fairly pure epithelial population.
Posted 03 September 2004 - 12:39 AM
I have a specific tissue (nose epithelial) I want to make the cultures from, and they have a limited life span, so I think by this method I'd just get the isolated and then they'd die.
Can I make use of serum free media that enhances growth of epithelial cells and then check them with anti vimentin antibodies, or is there a better method. I have found a method where they place the minced tissue in media + serum for a while, then remove the tissue, trypsinize, and place it in the special media (DMEM/Ham's F12, Nasal epithelial cell growth medium(oligene) or Airway epithelial cell growth medium(promocell)). It sounds a bit too easy?
Are there anybody who knows anything about these media and protocols for establishin primary epithelial cells.