Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Understanding the calculation

Calculation antibody calculation

  • Please log in to reply
1 reply to this topic

#1 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 255 posts
15
Good

Posted 15 February 2017 - 11:10 AM

Hi, 

 

I'm bit lost and want to know if I'm doing it right. 

I have an antibody and the data sheet says use it at 1-5 µg/ml. Does this mean that the antibody needs to be diluted between 1:1000 - 1:5000?

 

Thanks.



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,674 posts
565
Excellent

Posted 16 February 2017 - 06:47 AM

Depends on the concentration of the antibody. Have a look at the data sheet and tube, there should be a concentration on one or both of them. Hopefully it will say something like 1 mg/ml in which case a 1:1000 dilution will give you 1 ug/ml, and a 1:200 will give you 5 ug/ml.







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.