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checking wild type strain of restriction system

how ?

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#1 lactamase



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Posted 15 February 2017 - 02:23 AM

Hello all,


I am manipulating an wild type gram negative bacteria (proteus sp), and would like to transform it with an vector for reducing the expression level of cell division protein.


However, I am not sure whether this wild type bacteria have restriction endonuclease system or other system that hinder the transformation.


Shall I knock out those restriction endonuclease system first before my transformation? Further how could I know what type of restriction endonuclease system it have if there is no full genome sequence / publication for this strain?


Thank you very much 





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