Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Counting and seeding cells?].


  • Please log in to reply
1 reply to this topic

#1 vicky_311

vicky_311

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 13 February 2017 - 10:20 AM

hi! I am new cell lines and counting and seeding, so I need help, please.

I put the trypnize cell in 4 ml of medium and took 100ul of dilution and 100ul
of trypan blue and put in Neubauer Chamber and count in 5 squares and the I got average 52.1 cells

so, now I want to seed 70000 cell/ml in each well (24 well plate), could you
help me with the next steps, please. I need to put 0.5ul n each well

 

thank you

 



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,362 posts
509
Excellent

Posted 14 February 2017 - 09:41 AM

have a look here: http://www.protocol-...indpost&p=55266






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.