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Counting and seeding cells?].

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#1 vicky_311



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Posted 13 February 2017 - 10:20 AM

hi! I am new cell lines and counting and seeding, so I need help, please.

I put the trypnize cell in 4 ml of medium and took 100ul of dilution and 100ul
of trypan blue and put in Neubauer Chamber and count in 5 squares and the I got average 52.1 cells

so, now I want to seed 70000 cell/ml in each well (24 well plate), could you
help me with the next steps, please. I need to put 0.5ul n each well


thank you


#2 bob1


    Thelymitra pulchella

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Posted 14 February 2017 - 09:41 AM

have a look here: http://www.protocol-...indpost&p=55266

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