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How large need homology arms to be?

CRISPR/Cas9 Homology Directed Repair homology arms

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#1 Luca

Luca

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Posted 07 February 2017 - 08:33 AM

I would like to insert a 2808 sequence bihind a gene by CRISPR/Cas9 homology repair. Are 800 bp-homology arms large enough or should they be larger?

Thank you

LP



#2 bob1

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Posted 08 February 2017 - 11:41 AM

800 bp is usually enough for these sorts of things. In theory you should be able to get away with  much shorter - as little as 100 bp possibly.







Also tagged with one or more of these keywords: CRISPR/Cas9, Homology Directed Repair, homology arms

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