So that may be a dumb question, but I want to be really sure about that.
I've prepared 500ml LB agar medium yesterday and added ampicillin after the autoclave program, when the solution was at 50 degrees. I did not use all of the LB agar (containing Amp), so I'm currently storing it in the fridge.
Of course it's solidified and I know, that I can heat it up again in order to pour new plates.
My question is: Can I assume, that all of the ampicillin is degraded after 5 min of microwaving? I think it is not heat-stable at all, so I should be fine adding the amount of ampicillin, which I would normally add, if there was no antibiotic added before. Do you think I'm on the safe side with that or do you think there might still be some residual Amp present in the LB agar?
Your help is highly appreciated!