Hi all,
I've been doing some transfection of a plasmid containing Luciferase sequence. After the protocol for lysis of the cells from the plate, I freeze them on -80oC and keep it 'till the luminometer is available (I'm using one from a colleague's lab and I have to schedule the use with some anticipation).
My question is: for how long can I keep the cell lysates frozen without any loss on luciferase activity?
The maximum I've kept was 3 days with no losses, but now I'll have to wait (much) longer.
Thanks.
