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Freezing cell lysate for later Luciferase Assay

transfection luciferase assay lysate freeze

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#1 matheusmlot

matheusmlot

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Posted 18 January 2017 - 04:55 AM

Hi all,

 

I've been doing some transfection of a plasmid containing Luciferase sequence. After the protocol for lysis of the cells from the plate, I freeze them on -80oC and keep it 'till the luminometer is available (I'm using one from a colleague's lab and I have to schedule the use with some anticipation).

 

My question is: for how long can I keep the cell lysates frozen without any loss on luciferase activity?

 

The maximum I've kept was 3 days with no losses, but now I'll have to wait (much) longer.

 

Thanks.

 

 







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