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Plant Genomic DNA Extraction Kit ?


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4 replies to this topic

#1 dinesh_nato

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Posted 23 August 2004 - 01:17 AM

Hi I need to extract genomic DNA from plant

Organism : Chilli aka Hot pepper , Capsicuum annum

Downstream applications : PCR , Inverse PCR and Digesting , Sequencing

Sample size : approx. 1g or less

Any advice on what kit should i use (Which company ?) ...

Thanks in advance
;)

Edited by dinesh_nato, 23 August 2004 - 01:21 AM.


#2 bitpas

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Posted 23 August 2004 - 07:19 AM

Hi

"Nucleon Phyto Pure" from Amersham Bioscience

gives me allways very nice amounts and good DNA quality

:rolleyes:

#3 trinoceronte

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Posted 23 August 2004 - 10:51 AM

Hi:

Qiagen products also work well for plant DNA purification.
Be sure you try to use softer leaves (younger) that will provide better yields.
Our lab used both (Amersham and Qiagen) as kits for Capsicum annuum DNA purifications, both worked well. Also CTAB protocol and Dellaporta et al work fine. Depends on your access to kits.
Good luck ! :rolleyes:

#4 lyrezxl

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Posted 23 August 2004 - 05:59 PM

:rolleyes: Qiagen works very well here.

#5 dinesh_nato

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Posted 24 August 2004 - 01:16 AM

Thanks a lot everyone :rolleyes:




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