Hi all, I need help with the following.
I want to get a mutated protein expressed in plants.
If I have the relevant nucleotide sequence, how can I get a new DNA sequence TO A TUBE, without commercially synthesizing it?
Are there any vectors which can be used both for cloning and
overexpression of plant proteins?
I came across pB2GW7 vector. The literature says that it does not have a tag. Can we add the tag to the primer or is there any other way to purify the protein produced if its produced with out a tag like 6 His.