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Increasing lipofection efficiency by agitation?


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#1 seanspotatobusiness

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Posted 16 January 2017 - 08:10 AM

Has anyone else tried this? For me, agitation did not improve efficiency for Lipofectamine 3000 or DMRIE-C. There's a magazine article about it here but I couldn't find much else about it online with my lazy, half-assed search.



#2 bob1

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Posted 17 January 2017 - 02:03 PM

Interesting... I would suggest that their 1% was due to poor technique and that agitation would not dramatically increase the % to 80, sounds like something else is in play here. I did find a paper (1998) that suggests that agitaion of the complexes reduces stability.

 

I have routinely used the lift cells and transfect during plating method with good results, but it pays to check that the plasmid doesn't affect attachment of the cells.






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