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vector selection

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#1 Chomolungma



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Posted 14 January 2017 - 02:14 AM

Hi all, I need help with the following..


I want to get a mutated protein expressed in plants.


If I have the relevant nucleotide sequence, how can I generate the new sequence of DNA.

Are there any vectors which can be used both as a cloning vector and expression vector?


I came across the pB2GW7 vector. can anyone add a link to download the vector map of this? and the literature said this vector does not have the tag. since I need to purify the protein by chromatography, (preferably nickel nta method)is it ok to use this?


Please clarify and thank you



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