Edited by Dima, 02 January 2017 - 01:46 AM.
How to calculate phenols from gallic acidTPC determination
Posted 02 January 2017 - 01:45 AM
Posted 03 January 2017 - 03:05 PM
You do this just the same as you would for say a BCA assay or any standard curve - you take the absorbance and put it into the equation generated by the standards and solve to give you concentration. Just be aware of the usual precautions - don't extrapolate; dilute unknowns so that the absorbances lie within the range the standard curve. Make sure you are fitting the right sort of curve. Many graphing programs will do the calculations for you if needed.
To calculate the concentration of the phenolics, you would need to the proportion of phenolics to other organics, then work from there.
Posted 04 January 2017 - 02:55 PM
Edited by Dima, 04 January 2017 - 02:57 PM.