I'm working with the MAX Efficiency® DH5α™ Competent (cat num 18258012 from Life Technologies). I bought 1 kit because originally that was what I needed, now more vectors are needed to clone and will end short from material. This may sound a silly question, but I haven't work with cloning for about 15 years. If I propagate the bacteria on LB Broth I have to make them chemically competent again? Thanks in advance for any help!