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does anyone know real-time methyaltion pcr?

Started by hslab, Aug 21 2004 06:27 PM

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#1 hslab

hslab

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Posted 21 August 2004 - 06:27 PM

I want to design a pair of primer for the DLC-1 tumor suppressor gene and the probe, I can find the primer sequence according the reference, but how can I design the probe, please help me with it

thanks

Ming

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Posted 27 August 2004 - 05:41 PM

probe design is not a big deal. use your modified DNA as template and follow probe design rules for regular real-time PCR.
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