Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Anti-CD3 stimulated IL-2 cytokine production (splenocytes)

Splenocytes IL-2

  • Please log in to reply
No replies to this topic

#1 Mireille

Mireille

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 25 November 2016 - 05:09 AM

 I wanted to quantify the levels of IL-2 in spleen cells.  Therefore I performed an ELISA, but the results were not as I expected, the non-stimulated cells had higher levels of IL-2 than the stimulated ones.

My question is, could be another reason, besides a problem with the stimulation solution, that caused this effect on the stimulated cells?

Could be a problem with the lysis buffer?






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.