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Reversing Formaldehyde crosslinking

formaldehyde reversal crosslinking

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#1 Jen

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Posted 22 November 2016 - 02:16 PM

Hi, 

I am trying to reverse formaldehyde crosslinking and cannot find a generic protocol. I did see a suggestion of using 1 ul of 5 M NaCl to 25 ul of sample and incubating at 65 degrees overnight. Is this a good way of doing the reversal? 

Thanks!



#2 bob1

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Posted 23 November 2016 - 06:54 AM

Crosslinks between what sorts of molecules? I'm not sure that protein cross-links can be reversed, but you might be able to do DNA/protein.



#3 Jen

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Posted 23 November 2016 - 08:01 AM

It is protein-protein crosslinks... Isn't formaldehyde crosslinking reversible no matter what molecules were crosslinked though?


Edited by Jen, 23 November 2016 - 08:03 AM.






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