Recently, the flow cytometer (BD FACSort) that I am using (with other labmates) is having some problems.
The Drain, Fill and Backflush functions are working fine. However, the Run function is not working properly.
When the vent valve of the sheath fluid is closed, liquid is not taken up by the machine, and even there is some leakage from the machine into the sample tube. However, if the vent valve is opened, liquid can be taken up.
We tried using a wire to clean up any blockage in the acquisition tube. It did solve the problem for a while but the problem came back during the washes with bleach solution right after that.
Can anyone suggest any solution to solve the problem?