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quick dilution question urgent


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#1 molbio1234



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Posted 15 November 2016 - 08:20 PM

I have made up a dilution of 0.5ng DNA in 2.5ul total volume. I take 1.25ul of this and add it into a 5ul total reaction volume. What is the input into the reaction?


My answer is 0.25ng (I took 1.25ul, which is half of 2.5ul, making it also half the amount I took). Is this correct?

Edited by molbio1234, 15 November 2016 - 08:22 PM.

#2 mdfenko


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Posted 16 November 2016 - 04:34 AM

yes, but do you want to determine the final concentration of dna?

talent does what it can
genius does what it must
i used to do what i got paid to do

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