I want to calculate the whole body amount of a specific toxin by analyzing the excreted toxin in urine. I know that there are assumptions that are necessary like the toxin needs to be in steady state and so on.
But there are several Problems.
- First it is best if you use urine to get a 24h urine and if not possible you need to korrekt it by the creatine concentration which we want to do.
- I have the more or less normal half life time of the toxin at about 58 days. Well brain e.g. has a longer half life time as compared to blood but the median ist about 58 days. I am not sure if this is right because the data is from the early 70' and 80' so I want take into account different half life times.
- The toxin appears in different subforms as inorganic and organic subforms that are differently distributed through out the body. I know that I could use 3 compartment approaches but never did this before.
- I found old data that compare the blood to total daily toxin uptake by e.g. 0.25ng/ml blood equals 2.5 µg/day. These data is also very old and I am not sure it is correct.
So I can tell me somebody how this works or give me a link to some good literature?
Thx so much