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Question of Toxin and cell numbers in same solution volume


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#1 Montys

Montys

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Posted 04 November 2016 - 04:51 AM

Hi,

 

I am curious about the possible problems that occur if you for example put 50ng of HG₀ in a specific volume of cell culture but with different amount of cells in each.

 

So in one dish you have 10³ cells and in the other dish you have only 10 cells both dishes are filled with 2ml. Now you add 50ng HG₀.

How can you best explain the differences and problems regarding the Toxin?  You could say the cells in one dish gets 10 molecules of toxin/cell and in the other there are mIllions molecules/cell.

 

But isn't it to easy because not all toxin molecules will hit after a certain time period the cells. 

How can you explain this to people, because normally on says if you have the same concentration it does the same harm.

 

Sorry but right know I don't know how to explain it in a better way.

 

Thanks Monty






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