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DNA decontamination chemistry

DNA Decontamination DNA Erase

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#1 squallweathered

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Posted 04 November 2016 - 01:37 AM

Hi all,

 

Could anyone point me to an explaination of the chemistry behind the use of 8% phosphoric acid in commercial DNA decontamination sprays? For example the Look Out DNA Erase spray from Sigma states that the active ingredients are a surfactant and 8% phosphoric acid.

 

Does the phosphoric acid break the DNA down to mono-nucleotides? Or even further?

 

Many thanks to all for any information!



#2 El Crazy Xabi

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Posted 07 November 2016 - 02:36 PM

Actually, according to this paper, it might actually be useless. They tested a different phosphoric acid-based DNA decontamination agent (DNA Remover, now PCR Clean), though. Worth a read anyway.

https://www.ncbi.nlm...les/PMC4943653/






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