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Protocol for human dendritic cell population in blood


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#1 Shannah

Shannah

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Posted 03 November 2016 - 01:42 AM

Hi,

 

I'm new to Flow cytometry and I'm looking for a protocol to stain and gate human  dendritic cells from blood using flow cytometry. The Panel should incluce CD86, CD40 and maybe CD80, we have BD LSRFORTESSA so the Panel can incluce a lot of Colors. I wasn't able to find a paper using a Panel that distinguis between pDC and cDC and further on HLA-DR, Cd86 and CD40.

 

Maybe someone has a protocol for that and would like to share.

 

Thank you.






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