Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Cryopreserving human ES directly in 24-well plate

  • Please log in to reply
No replies to this topic

#1 neuropath



  • Active Members
  • PipPipPipPipPip
  • 38 posts

Posted 31 October 2016 - 11:50 PM

Hello Bioforumers,


I have several 24-well plates of CRISPR knockout human ES clones that I don't have time to screen for the next couple of weeks. I am hoping to freeze them down in the plates until I have time to screen them. They are growing feeder-free in TeSR-E8 medium at the moment. Does anyone have a good protocol to do in-plate freezing? I have found some protocols online but they all involve adding FBS to the freezing medium. My ES cells have not been exposed to FBS for many passages and I do not know how exposure to FBS again will affect them. Can KOSR be used as a substitute for FBS in the freezing medium?


Thank you for any suggestions or thoughts.


Kind regards,


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.