I was wondering if anyone has any experience with already designed qPCR primers? I've only ever designed my own ones when I was working with zebrafish and the process of testing and validating the primers before I can even use them for the real assay was very laborious and time consuming. I'm currently working with human samples now and OriGene offers predesigned, and I'm going to assume pre-tested, qPCR primers so I'm hoping it will cut down on the time I would need to test and validate the primers.
Does anyone have any experience with this kind of thing? If so, how was your experience?