genomic DNA digest
Posted 20 August 2004 - 07:59 AM
Posted 24 August 2004 - 06:32 AM
BTW, you only use 500ng DNA for the Southern? What kind of labelling method do you use? I had trouble while using DIG for mouse genome Sothern. I usually use more than 20ug DNA. I am wondering whether I used too much DNA.
Edited by msshiao, 24 August 2004 - 06:35 AM.
Posted 24 August 2004 - 11:26 AM
Most protocols I've seen use at least that much and sometimes more.
I've been told to make sure the probe works well first with standard P32 protocol. Then try DIG labelling.