Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

IHC Protein Competition, Protein Background

IHC Protein Competition Background Blocking

  • Please log in to reply
1 reply to this topic

#1 Laura Robin

Laura Robin


  • Members
  • Pip
  • 1 posts

Posted 12 October 2016 - 12:25 PM

Hi there,


I am attempting to perform a protein competition with a commercial antibody. The signal from the antibody seems to have been reduced, but we cannot say definitively as there is intense background from the protein on the tissue. 


I was hoping someone could recommend me the best course of action in order to eliminate this background. I have tried BSA, but this eliminates staining from my antibody.


Thanks for your help!

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,670 posts

Posted 13 October 2016 - 01:14 PM

There are a range of different buffers you can use for antibody binding solutions (other than tris and phosphate based buffers), and also a range of different blocking reagents you might try (casein, gelatin (fish, pig, cow), normal antisera, albumin etc.). 


Some details on your protocol might help.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.