Hi I wondering if anyone out there could help with this.
I'm looking for autoantibodies in a healthy group of patients Vs disease group.
I've done some immunoprecipitation using IgG extracted from patient blood. I ran the IP out on a 2D gel and then had the spots identified by mass spectrometry. Our results indicate that there are a lot of albumin isoforms on the 2D gel and that they are reacting with the IgG.
Indicating that the patients are producing antibodies against these various isoforms of albumin.
I need to verify this to be sure. Most of the isoforms (based on the sequences identified by MS) seem to be missing their N-terminus.
In terms of buying in an anti-albumin antibody to verify my work - Would an anti-albumin antibody that has been raised against the entire sequence of albumin bind with cleaved isoforms of albumin? Or will it only ever bind to the full sequence?
Anyone have any other ideas how I go about verifying the MS results?