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AML primary cell mouse PDX transplantology problem

AML cancer primary cells immunotransplantology

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#1 DmitryM

DmitryM

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Posted 21 September 2016 - 07:23 AM

Dear colleagues,
 
I have a problem with PDX: childrens M4 AML, 95% of blasts (CD34-51%,CD117-85%,CD38-85%,HLA-DR-73%,CD33-95%,CD64-20%,CD11c-19%,CD13-95%,CD7-41%,CD4-33%). 2 NSG mouses were dead within 2-3 day after transplantation. Both transplantation were performed with different reagents and equipment.
 
Blood was with heparin. Blasts: 
f8778beb707834ef27539a5e687f8510.png
 
10E7 cells, in 0,4 ml PBS, via tail vein. 
 
Only 2 ideas:
 
1) Warfarin premedication must be applied
2) Blood non sterile
 
Could anybody some help? 
 
Regards, 
 
D. 
 

 






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