I need to perform a cell culture based assay, wherein I need to grow cells in presence of drug and test the effect of that on the cells. This has been already done by someone in the lab. I need to reproduce the results. The problem is, the assay was done in 15cm dish and that is not currently available with us. I need to do this experiment ASAP. We have T75 flasks, and surface area to grow cells is half of 15 cm dish.Therefore I am planning to do exp with 2 T 75 flasks.
Can drug concentration given for 15cm dish be used for T75 flask also? Or do I need to make it half?
I have calculated seeding density accordingly, however I am confused about drug conc. Eventually I have to pool both the flask to get same number of cells to make lysate.
Somebody please help me, so that I can put up the experiment.