I plan to conduct cell invasion assay on my stable cells in the future. I've never done this assay and would appreciate any advice.
I would like to know how do I know what type of basement matrix to chose prior to ordering the assay kit i.e. collagen I invasion assay, lamini I invasion assay etc. Do I do a qPCR to analyze the changes in various extracellular membrane proteins prior to ordering the assay with the appropriate matrix to test?