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cell invasion assay


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#1 SF_HK

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Posted 18 September 2016 - 08:51 AM

Hi,

 

I plan to conduct cell invasion assay on my stable cells in the future. I've never done this assay and would appreciate any advice.

 

I would like to know how do I know what type of basement matrix to chose prior to ordering the assay kit i.e. collagen I invasion assay, lamini I invasion assay etc. Do I do a qPCR to analyze the changes in various extracellular membrane proteins prior to ordering the assay with the appropriate matrix to test?

 

Regards

SF

 

 



#2 ibmbio

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Posted 26 September 2016 - 02:55 AM

There are plenty of publications giving details about how and what you need for the invasion assays - you can find videos as well.

 

For the commercially available plates, the basement usually coated with matrigel, which is a mixture of the ECMs - that typically includes collagen and some other materials. 

 

Sorry, but I can't see any obvious purpose to do the qPCR before doing the assay, unless you have treatment - so you are comparing treated and untreated cells after/before the invasion assay.






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