Hello I am writing from Peru. Currently I am working in a Flow cytometry laboratory in oncohematology. The question is that we work with the Euroflow consensus and of course we work with manufacturers, clones, quantities includes in its protocol, the question is that we want to change the manufacturer (the mayority of reagents and solutions are BD) in our panels, so we start with some antibodies and we noticed that in some other manufacturers, using the same clone, the same quantity, and the same fluorescence we found that the MFI in some antibodies are dicreased in comparison with the clones and marks that we actually use in laboratory. Another issue is in the use of red blood cell lysing, we found that some subsets are lower than when we use the actual lysing. And a finally question, is incorrect to say " that reagent doesn't work" because all manufacturers in the world invest to produce and sell their product, that of course have to work. But why are that kind of differences, the method doesn't reproduce in my lab, althought I follow their protocol and Technical data sheet step by step.
I hope someone can help me. I am really desperate.
Thanks a lot