5X, 6X sds gel loading buffer
Posted 16 September 2016 - 11:35 PM
Posted 19 September 2016 - 03:37 AM
i've never had 10% sds crash out at room temperature.
maybe the vessel or one of your stock solutions is contaminated with a cation that forms a precipitate with sds?
talent does what it can
genius does what it must
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Posted 20 September 2016 - 06:53 PM
10% SDS in 50% glycerol (i.e. 5x sample buffer) has always crashed out in every batch I've made for the last 15 years. I assume this is why it's hard to buy 5-6x loading buffer with this formulation. Most commercial 5x recipes that I've seen have either less glycerol or less sds.
10% sds in water usually shouldn't crash out, but can on occasion...depends on manufacturer and, as you suggested, probably purity.
Non-issue after a few seconds of microwaving tho.
Edited by labtastic, 20 September 2016 - 07:09 PM.
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Posted 28 September 2016 - 06:46 AM