I performed Multiplex PCR in WGA amplified products with SurePlex kit and in my results i found a relationship between GC content and PCR Efficiency and ADO.
The GC content of my Pcr products ranges between 26% and 50%. In my results i found that PCR products with higher GC content (50%-45%) had better PCR efficiency than pcr products with lower GC content (26%-34%).
I know from the bibliography that PCR products with high GC contend (>50%) tend to have lower percentage of PCR efficiency. I try to interpret my results , i was wondering if the annealing of primers used in SurePlex in AT rich regions is unstable and so the PCR efficiency of these regions is lower.
is this theory logic?