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Whole Genome Amplification and GC content

WGA SurePlex

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#1 Lay La

Lay La


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Posted 09 September 2016 - 09:54 AM



I performed Multiplex PCR in WGA amplified products with SurePlex kit and in my results i found a relationship between GC content and PCR Efficiency and ADO.


The GC content of my Pcr products ranges between 26% and 50%. In my results i found that PCR products with higher GC content (50%-45%) had better PCR efficiency than pcr products with lower GC content (26%-34%). 


I know from the bibliography that PCR products with high GC contend (>50%) tend to have lower percentage of PCR efficiency. I try to interpret my results , i was wondering if the annealing of primers used in SurePlex in  AT rich regions is unstable and so the PCR efficiency of these regions is lower. 


is this theory logic? 

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