Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Whole Genome Amplification and GC content

WGA SurePlex

  • Please log in to reply
No replies to this topic

#1 Lay La

Lay La

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 09 September 2016 - 09:54 AM

Hello,

 

I performed Multiplex PCR in WGA amplified products with SurePlex kit and in my results i found a relationship between GC content and PCR Efficiency and ADO.

 

The GC content of my Pcr products ranges between 26% and 50%. In my results i found that PCR products with higher GC content (50%-45%) had better PCR efficiency than pcr products with lower GC content (26%-34%). 

 

I know from the bibliography that PCR products with high GC contend (>50%) tend to have lower percentage of PCR efficiency. I try to interpret my results , i was wondering if the annealing of primers used in SurePlex in  AT rich regions is unstable and so the PCR efficiency of these regions is lower. 

 

is this theory logic? 







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.