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Live tissue sectioning

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#1 moerae



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Posted 09 September 2016 - 03:26 AM

Hi all,


I don't quite know where to post this so I've put it here. I'm working with patient skin biopsies, which we'd like to section very thinly so we can do some work on it. We'd like to section it fresh and unmounted; however, the samples are very squishy and I'm scared it'll go everywhere when the blade presses down on it. Does anyone know of a way to "fix" it without killing the sample so we can section it and free it from the "fixative" for subsequent work?


I was thinking agarose/agar but if anyone has experience with this or ideas will greatly appreciate the input and advice!



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