I have been trying (unsiccesfully) to extract DNA from horse hair samples.
I have tried the Qiagen user developed method, and have had rubbish levels of DNA back (around 2ng/ul). I have recently tried another method using proteinaseK, Tween20, NP40, promega taq buffer and MgCl2 extracted for 60C 45mins, then 95C for 15mins.
I have got the following curve on the nanodrop... really high peak at 230nm and a shift in the DNA peak from 260-280
would cleaning up the DNA work?
Any advice or suggestions greatly appreciated.