I know that this topic was covered here and there but I have an issue and can't get my head around the maths, any help would be really appreciated.
I've done a normal ChIP but instead of taking 1/10 of the chromatin as input, I evenly split the chromatin between the input and my samples.
1/3: H3K4me3 IP
1/3: H3K27me3 IP
I've processed it all downstream, and re- suspended my pellets in the same volumes of water, and then went on to dilute the samples to the same concentration so I can run a qPCR on them. But the results i'm getting dont make sense and I think its because I don't know how to take the % input into account.
Can anyone walk me through it based on the above info?