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ChIP-qPCR, calculating % input

ChIP qPCR ChIP-qPCR

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#1 Valie15

Valie15

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Posted 02 September 2016 - 06:11 AM

Hello,

 

I know that this topic was covered here and there but I have an issue and can't get my head around the maths, any help would be really appreciated.

 

I've done a normal ChIP but instead of taking 1/10 of the chromatin as input, I evenly split the chromatin between the input and my samples.

 

1/3 Input

1/3: H3K4me3 IP

1/3: H3K27me3 IP

 

I've processed it all downstream, and re- suspended my pellets in the same volumes of water, and then went on to dilute the samples to the same concentration so I can run a qPCR on them. But the results i'm getting dont make sense and I think its because I don't know how to take the % input into account.

 

Can anyone walk me through it based on the above info? 







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