we came to a point when applying qPCR in-house protocols, in our lab,
to need to generate our own exogenous positive controls and internal controls too.
we are dealing with viruses to be detected in animal tissue samples.
what's the best internal control -as from your experiences- to be used ?
and we need to develop a protocol in which we detect the "pathogen" and the "internal control"
in the same reaction,
i.e : to put the primers and probes for both the pathogen and the internal control
all in the same tube ...
making it easier for the work load.
you know any guides i should follow in this regard ???
thanks in advance,
Edited by nightingale, 24 August 2016 - 10:35 PM.