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SDS PAGE gel length effect on resolution


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#1 biotef

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Posted 22 August 2016 - 03:41 AM

we perform SDS page of protein with 12-15% gel. We are currently using gel length of 10 X 10 Cm and are planning to switch over to a new system that has its dimension of 8.3 X 7.3 cm. Kindly guide me what will the effect be on the bands resolution / separation on reducing the gel dimension. thanks in anticipation megha

#2 mdfenko

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Posted 22 August 2016 - 01:32 PM

your bands will be less separated but their Rfs will remain the same (if length is the only difference).

 


Edited by mdfenko, 22 August 2016 - 01:33 PM.

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