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How to identify the genomic target region for positive control in ChIP assay

ChiP

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#1 Sandeep Gupta

Sandeep Gupta

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Posted 17 August 2016 - 05:47 PM

Hello All, 

I am a beginner in the field of genomics/ proteomics but now I have to do ChiP-Seq analysis for my work. Before going to the sequencing part, I first wanted to confirm whether general ChiP technique is working in my hand. I know the genes which are known to be targeted by my transcription factor (positive control gene) but I don't know how to select the genomic region where they bind, and design primers which can selectively amplify these regions after the ChiP. I would really appreciate any help/suggestion. 

Also, if anybody knows good ChiP kit, please let me know too. 

 

Thanks a ton,... 

 

 

Best 

 







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